Common Microorganisms Essay examples -- essays research papers

INTRODUCTIONMicroorganisms much(prenominal) as bacteria, fungus, mold, and barm are present and common in roughly every environment on earth. The normally microscopic organisms sess con arrayrably be seen using differing types of agar, which creates an ideal environment for the organisms to form colonies, which are groups of hundreds of organisms that can be seen with the naked eye. In order to see individual microorganisms, it is needed to use the magnification of a high-powered microscope.These techniques of microbiology are use in the following five samplings. The first experiment used Trypticase Soy solid food Agar (TSA), which can grow a wide variety of organisms and contains casein and soybean meal and a minute NaCl, to study the effectiveness of intoxicant as a skin antiseptic. The second experiment tested the effectiveness of different kinds of mouthwashes as antiseptics using TSA as well. Experiment number common chord explored the normal human flora existing on skin and in nasal cavities, and two types of agar were used, including TSA and mannitol Salt Agar, which contains manitol sugar, phenol red, and 7.5% NaCl. mannitol Salt Agar tests for the presence of staphylococci bacteria that can last in the salt that inhibits the growth of most other bacteria. Some forms of staph bacteria ferment mannitol and produce a yellow discolor around the colonies, which can easily be seen against the red background. The fourth experiment studied the number of bacteria in a diluted sample of uncooked hamburger meat using intellectual nourishment agar and a Quebec counter to count the colonies. The final experiment involved the growth of yeast cells under aerobic and anaerobic conditions. MATERIALS - Trypticase Soy Nutrient Agar-mannitol Salt Agar-Sterile alcohol swab-Listerine mouthwash-Tubes of nutrient agar-Diluted hamburger meat -Quebec colony counter-orchard apple tree juice-Anaerobic yeast culture-Hemacytometer-IodineMETHODSExperiment 1. militar y capability of alcohol as an antiseptic1) All experiments must be make in a sterile environment Lysol can be used to sterilize the a... ...ide had a lot of large tan colonies and dense, small colonies. The Listerine side seemed to destroy all most all of these small colonies, but the larger colonies were actually more numerous. The other mouth washes used were salt piss rinse and mountain chain. The salt water did almost no damage to the bacteria, and the Scope killed the most. In experiment three, there were a lot of different kinds of organisms persent on the agar. On the scale leaf that contained nasal micro-organisms, large yellow colonies appeared on the Mannitol Salt Agar, indicating Staphylococcus aureus. On the TSA side, small creamy white colonies were besides present. These could possibly be yeast. On the plate that contained the skin swab, there was a large amount of large, fuzzy white and black growth, which is mold. There was a single light-orange colony on the TSA side, possibly a growth of Flavobacterium.In the fourth experiment, there were a number of different kinds of colonies on the EMB plate with the meat swab. There were black dots, which hint the presence of E-coli, pink dots, which indicate lactose-fermenters, and a few white and gray colonies.